Results of IHC staining in patch-, plaque-, and tumor-stage MF. (A) TIMP1, (B) RAC2, (C) SMA, (D) FAP, (E) DCN, and (F) CD163. IHC staining intensity was rated on a scale of 0 to 3 using a semiquantitative method. The ratings were as follows: 0, 1, 2, and 3, indicating <10%, 10% to 30%, 30% to 50%, and >50%, respectively, of the total area of interest (magnification ×200; scale bar, 200 μm).