Digital spatial transcriptome analysis revealed that transcriptomic expression differed among patch-, plaque-, and tumor-stage MF. (A) A dimension reduction plot of UMAP showed that patch- (marked in yellow), plaque- (marked in blue), and tumor-stage MF (marked in red) form distinct clusters in the lymphoma cell area. (B) Unsupervised clustering of all genes revealed similar results. Volcano plot showing DEGs with fold changes >2 and P < .05 (C) and heatmap of DEGs (D) in lymphoma cell areas between patch- and plaque-stage MF. Volcano plot showing DEGs with fold changes >2 and P < .05 (E) and heatmap of DEGs (F) in lymphoma cell areas between plaque- and tumor-stage MF. Venn diagram showing common and distinct upregulated (G) or downregulated (H) DEGs among patch-, plaque-, and tumor-stage MF. PT, patient.