Transcriptomic analyses identify lymphoma-derived IL-10 as key immunomodulator of DLBCL microenvironment. (A) Volcano plot of differentially expressed genes between pBIC^Δ10 vs pBIC FACS-sorted B220⁺GFP⁺ lymphoma cells (supplemental Table 2). (B) GSEA comparing pBIC^Δ10 and pBIC lymphoma cells, highlighting significantly enriched and depleted gene signatures (supplemental Table 3). Functional categories are displayed and grouped by color, based on differential expression analysis of RNA-seq data. (C) Gene signatures summarizing the functional categories found in panel B (supplemental Table 4) were used in single-sample GSEA (ssGSEA) and correctly cluster pBIC and pBIC^Δ10 mice. (D) t-distributed Stochastic Neighbor Embedding (t-SNE) graph of an integration of 18 343 cells from 1 representative pBIC and pBIC^Δ10 murine lymphoma. (E) Mosaic plot displaying the distribution of cell types defined by scRNA-seq in pBIC vs pBIC^Δ10 lymphomas. The width of each cell type bar represents the proportion of that cell type within the total sample population, whereas the height indicates the relative frequency of that cell type between the 2 lymphoma models. NES, normalized enrichment score.