Single-cell RNA-sequencing (RNA-seq) and ATAC-seq analysis of differentiating hPSCs and CB CD34⁺ cells. (A) Schematic diagram of differentiating hPSC isolation. (B) ForceAtlas2 (FA) plots show cell clusters. The plot on the right shows the distribution of cell sources among the clusters. (C) Scorecard plot showing the selected hematoendothelial and arterial genes. (D) Feature FA plots showing expression of indicated hematoendothelial genes in all cell samples. (E) Heat map–dot plot showing transcription factor expression of high-quality eRegulon on a color scale and the RAS of the eRegulon on a size scale. (F) Feature FA plots and pseudotime of aggregated hPSC clusters. Pseudotime was generated by SCENIC+ from the area under the curve values of all RNA-seq and ATAC-seq eRegulons, with a root cell predicted from C6. (G) Feature FA plots of differentiated hPSC showing the cell clusters and the expression of genes associated with NOTCH signaling. (H) Violin plots showing the ATAC peaks with the largest changes between clusters at the NOTCH1 and NOTCH4 promoters and intronic regions. FACS, fluorescence-activated cell sorter; in14, intron 14.