CIMP, MRD, WBC, and outcome in T-ALL in the study of NOPHO ALL2008 cohort. (A) Heat map of the 1091 CIMP panel CpGs in n = 192 NOPHO ALL2008 T-ALL samples at diagnosis, classified as CIMP low (n = 76) and CIMP high (n = 116) in CIMP% order. Healthy sorted CD34⁺ and CD3⁺ T cells and lymph node samples are revealed for comparison. (B) Distribution of the outcomes in 172 patients in relation to D29 MRD, WBC, and CIMP methylation %. MRD is log₁₀ transformed, and WBC is zero scaled at 50 and log₁₀ transformed. Yellow lines represent MRD 0.1%, WBC 50, and CIMP 40% cutoffs. (C) Alluvial plot depicting the timeline of patients from CIMP subgroups at diagnosis to the D15 MRD and D29 MRD until outcomes. In panels B and C, only the 172 patients with complete CIMP, WBC, and MRD data are illustrated. NS, not stratified due to IF.