Figure 3.
IFN-γ and TNF sensitize AML cells to STING agonism. (A-B) Levels of IFN-α2a (A) or CXCL10 (B) measured after 72 hours by CBA (IFN-α2a) or ELISA (CXCL-10), from cocultures of human T cells and HL-60 cells treated as indicated (E:T, 1:10; n = 3). (C) Levels of CXCL-10 measured after 16 hours in the supernatant of HL-60 cells treated as indicated (IFN-γ, 20 ng/mL; TNF, 0.5 ng/mL; cGAMP, 40 μg/mL). (D) Immunoblots of lysates of HL-60 cells treated for 16 hours as indicated (IFN-γ, 20 ng/mL; TNF, 20 ng/mL; cGAMP, 40 μg/mL). Means ± SEM are presented. Statistical analysis was performed using the ordinary 1-way ANOVA with the Tukey comparison or the 2-way ANOVA with the Tukey comparison (panel C). ns, P > .05; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. ELISA, enzyme-linked immunosorbent assay; ns, not significant.

IFN-γ and TNF sensitize AML cells to STING agonism. (A-B) Levels of IFN-α2a (A) or CXCL10 (B) measured after 72 hours by CBA (IFN-α2a) or ELISA (CXCL-10), from cocultures of human T cells and HL-60 cells treated as indicated (E:T, 1:10; n = 3). (C) Levels of CXCL-10 measured after 16 hours in the supernatant of HL-60 cells treated as indicated (IFN-γ, 20 ng/mL; TNF, 0.5 ng/mL; cGAMP, 40 μg/mL). (D) Immunoblots of lysates of HL-60 cells treated for 16 hours as indicated (IFN-γ, 20 ng/mL; TNF, 20 ng/mL; cGAMP, 40 μg/mL). Means ± SEM are presented. Statistical analysis was performed using the ordinary 1-way ANOVA with the Tukey comparison or the 2-way ANOVA with the Tukey comparison (panel C). ns, P > .05; ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. ELISA, enzyme-linked immunosorbent assay; ns, not significant.

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