![Effect of RSP and extended-stored CSP transfusion in healthy humans on ASA. (A) In each of the 2 periods, healthy humans underwent plateletpheresis with platelet storage (randomized to RSPs or CSPs). The healthy humans then received an ASA loading dose, 24 hours before transfusion, and autologous platelet transfusion with blood assessments at baseline (BL; ie, without ASA), before transfusion (ASA), and at multiple time points after transfusion (1 hour, 4 hours, and 24 hours). Participants completed an RSP period (receiving 7-day RT-stored platelets) and a CSP period (receiving 14-day 4°C-stored platelets) as outlined in this overview schematic. (B) The number of RT (red circles) and 4°C (blue circles) total platelets transfused. The platelet count (C) and corrected count increments (CCI) (D) during each period (red circles: RSP period; blue squares: CSP period; 4 hours, ∗P = .0204; 24 hours, ∗∗P = .0047). (E) Platelet AA–stimulated whole-blood aggregation was measured by VerifyNow ASA (1 hour, ∗∗P = .0055; 24 hours, ∗P = .0198). Maximum light transmission aggregation (defined as maximum aggregation recorded [in percent] within 10 minutes after agonist stimulation) in response to 0.5 mM AA (1 hour, ∗P = .0347; 4 hours, ∗∗P = .0081) (F), and collagen (2.5 μg/mL; 24 hours, ∗P = .0493) (G). The thrombin generation potential of the participant’s whole blood–derived PRP was measured immediately before and 1 hour after transfusion with a commercially available fluorogenic thrombin generation assay. Results are reported as the change from the pretransfusion value to the 1-hour posttransfusion value for thrombin generation peak (defined as maximum concentration during recording; ∗P = .0429) (H), start tail time (time until end of thrombin generation; ∗P = .0266) (I), time to thrombin peak (time between begin and peak of thrombin generation) (J), and lag time (time from assay start to begin of thrombin generation) (K). Data are shown as mean ± standard error of the mean (SEM), panels H-K shown with individual values. Unpaired data, n = 7 to 9, individual P values are shown in the text above. RT, room temperature.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/143/20/10.1182_blood.2023022593/4/blood_bld-2023-022593-gr1.jpeg?Expires=1752503425&Signature=D1gXpRJGEYQdM4ST0WMvG4gDghbY3ui612w~ctAMHRylgS-ZxQoSzgz0trEL2I0vePyC8cuk-fb2NhtXO6tROBRTLGS73DXHrMcbs1aa8O8hxK8gN25E~2WjSlyk9NwJfDj8xV~ERjiTof~Ur1mRRAiiI-u8txdkO4NB1D2wcjf2vFJuos~3x65W0T~DpNvRMEvrfnNUyuGuMKp5wUoVfAQ2C3~NzSXmqbUnqAdgtT2lHGdWMjGA2nWoPiqg~z4nlD~SbAOu0X3QDjYI7OsH0nLFlO-6S22KuNwH4AoL3YVT6s4eONOMVSOO-CR0wLddLQsL7fr26txMW8ZsEJqbQQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of RSP and extended-stored CSP transfusion in healthy humans on ASA. (A) In each of the 2 periods, healthy humans underwent plateletpheresis with platelet storage (randomized to RSPs or CSPs). The healthy humans then received an ASA loading dose, 24 hours before transfusion, and autologous platelet transfusion with blood assessments at baseline (BL; ie, without ASA), before transfusion (ASA), and at multiple time points after transfusion (1 hour, 4 hours, and 24 hours). Participants completed an RSP period (receiving 7-day RT-stored platelets) and a CSP period (receiving 14-day 4°C-stored platelets) as outlined in this overview schematic. (B) The number of RT (red circles) and 4°C (blue circles) total platelets transfused. The platelet count (C) and corrected count increments (CCI) (D) during each period (red circles: RSP period; blue squares: CSP period; 4 hours, ∗P = .0204; 24 hours, ∗∗P = .0047). (E) Platelet AA–stimulated whole-blood aggregation was measured by VerifyNow ASA (1 hour, ∗∗P = .0055; 24 hours, ∗P = .0198). Maximum light transmission aggregation (defined as maximum aggregation recorded [in percent] within 10 minutes after agonist stimulation) in response to 0.5 mM AA (1 hour, ∗P = .0347; 4 hours, ∗∗P = .0081) (F), and collagen (2.5 μg/mL; 24 hours, ∗P = .0493) (G). The thrombin generation potential of the participant’s whole blood–derived PRP was measured immediately before and 1 hour after transfusion with a commercially available fluorogenic thrombin generation assay. Results are reported as the change from the pretransfusion value to the 1-hour posttransfusion value for thrombin generation peak (defined as maximum concentration during recording; ∗P = .0429) (H), start tail time (time until end of thrombin generation; ∗P = .0266) (I), time to thrombin peak (time between begin and peak of thrombin generation) (J), and lag time (time from assay start to begin of thrombin generation) (K). Data are shown as mean ± standard error of the mean (SEM), panels H-K shown with individual values. Unpaired data, n = 7 to 9, individual P values are shown in the text above. RT, room temperature.
