ACLY silencing in primary PTEN-null T-ALL cells affects their viability. (A) pACLYser455 levels in 8 PTEN/PI3KCA altered and 6 WT PDX T-ALL blasts. (B) Representative FACS plot depicting pAktser473 and pACLYser455 in a PTEN/PI3KCA WT patient or a PTEN-deleted patient. (C) Percentages of GFP⁺PTEN–altered PDX blasts remaining viable 48 hours after transduction with lentivirus vectors carrying shRNA ACLY or a control construct. (D) Competitive transplantation assay, in which PDX cells transduced with a shRNA ACLY or a shRNA control competed with nontransduced cells in a 20:80 ratio following injection into NSG mice. The relative ratio of transduced and not transduced cells found in the bone marrow of NSG mice is shown. (E) Number of GFP⁺PTEN germ line PDX blasts remaining viable 3, 4, or 5 days after transduction. BM, bone marrow; FACS, fluorescence-activated cell sorting; GFP, green fluorescent protein.