Metabolic analysis of preleukemic PTEN-mutant thymocytes. (A) Principal component analysis of 4 Pten-mutant and 5 Ptenflox2 thymi issued from preleukemic 8-week-old mice subjected to unsupervised metabolic analysis. (B-D) Heat maps of the 50 most deregulated metabolites (B), glycolytic intermediates, Krebs cycle intermediates, glutamine, glutamate, citrate, CoA, acetyl-CoA, and fatty acids (C), and pathway analysis in 4 preleukemic PTENΔ/Δ thymocytes or 5 flox control counterparts (D). (E) Phosphorylated ser455-ACLY in the thymi of preleukemic PTENΔ/Δ mutant or control animals in 3 independent experiments, with at least 2 per group. Adj, adjusted; aKG, α-ketoglutarate; CoA, coenzyme A; F1,6DP, fructose 1,6 diphosphate; FAS, fatty acid synthesis; FDR, false discovery rate; TCA, tricarboxylic acid.²⁷