Figure 1.
Process development for generating 19-28z CAR EBV-VSTs. Cryopreserved EBV-VSTs (cultured for 28-35 days before cryopreservation) are thawed and stimulated weekly with irradiated B-LCL (days 0, +7, +14). T cells are transduced with 19-28z CAR at a cell density of 1 × 106 cells per mL and 0.5 × 106 cells per mL, respectively, on days +16 and +17. Cells are then fed and evaluated on days +18, +21, and +23 and then harvested and frozen on day +24.
