Lung IMs were increased at baseline and hypoxia-induced increase in lung IMs was prevented in Nbeal2^−/− mice. (A) Lung IMs were increased in Nbeal2^−/− mice at baseline compared with WT mice. (A-D) At day 3, total IMs and IM1, IM2, and IM3 subsets increased in WT but not Nbeal2^−/− mice. At day 21, total lung IMs and IM1s were increased in Nbeal2^−/− compared with WT mice. (E) Lung sections were stained with anti-CD31, anti-α-SMA, anti-CD68, anti-PF4, anti-CD41, and anti-CD45, and DAPI. Whole-slide scans were obtained using the Vectra Polaris spatial imaging scope (×40 objective, 0.5-micron resolution). Representative images of pulmonary vessels were captured. Representative image of IMs in WT and Nbeal2^−/− mice (original magnification ×40; scale bars, 50 μm). Statistics: P ≤.05 by 2-way ANOVA with Tukey post hoc analysis. Comparisons: (∗) WT compared with WT baseline, (#) Nbeal2^−/− compared with Nbeal2^−/− baseline, (^) WT compared with Nbeal2^−/−. HPX, hypoxia; NMX, normoxia.