Figure 3.
Functional characterization of CUB-NGLY variants. (A) FRETS-VWF73 activity (mean ± standard deviation, n = 3) of CUB-NGLY variants at pH 6 and pH 7.5. All activity values are relative to the activity of the variant at pH 6. (B) Conformation index of CUB-NGLY variants. Index is ratio of ADAMTS13 bound to the ELISA in the absence of 17G2 (an activating antibody) divided by amount of ADAMTS13 in the presence of 17G2 (n = 3). Significance was determined using ordinary 1-way analysis of variance using a Dunnett multiple comparison test, with a single pooled variance. ∗∗∗∗P < 0.0001. (C) VWF-multimer assay of wtADAMTS13 and CUB-NGLY variants. (D) Activity of wtADAMTS13 and CUB-NGLY variants under flow (n = 2). Platelet number percentage is relative to the number of platelets counted in the first frame.

Functional characterization of CUB-NGLY variants. (A) FRETS-VWF73 activity (mean ± standard deviation, n = 3) of CUB-NGLY variants at pH 6 and pH 7.5. All activity values are relative to the activity of the variant at pH 6. (B) Conformation index of CUB-NGLY variants. Index is ratio of ADAMTS13 bound to the ELISA in the absence of 17G2 (an activating antibody) divided by amount of ADAMTS13 in the presence of 17G2 (n = 3). Significance was determined using ordinary 1-way analysis of variance using a Dunnett multiple comparison test, with a single pooled variance. ∗∗∗∗P < 0.0001. (C) VWF-multimer assay of wtADAMTS13 and CUB-NGLY variants. (D) Activity of wtADAMTS13 and CUB-NGLY variants under flow (n = 2). Platelet number percentage is relative to the number of platelets counted in the first frame.

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