Increased platelet half-life fosters inflammation. (A) Schematic outline showing blood sampling from WT and BAK/BAX^plt–/– DKO. (B) Gating strategy for procoagulant platelets. (C) Bar graphs depicting flow cytometric analysis of percentage (%) of procoagulant platelets (P = .0121), P-selectin–positive platelet percentage (0.4077), PS⁺ platelet percentage (0.0092), and platelet size forward scatter area (0.0915). (D) Gating strategy for PNA in whole blood; bar graph shows the percentage of CD41⁺ aggregated to Ly-6G⁺ cells (P = .0031). Statistical tests for panels A-D, unpaired t tests, 2-tailed. (E) Experimental outline of acute lung injury in WT (n = 10), BAK KO (n = 8), BAX^plt–/– (n = 10), BAK BAX^plt–/– KO (n = 9), and rejuvenated BAK BAX^plt–/– KO (n = 4) after antibody-mediated platelet depletion. (F) Bar graphs showing flow cytometric analysis of PNA (P = .0010). (G) RBC count (P = .0017) and neutrophil counts (P = .0009) in BALF. For panels F-G, ordinary 1-way ANOVA with post hoc Dunnett multiple comparisons test compared with WT. (H) Assessment of cytokine measurements in BALF, 2-way ANOVA comparison between mouse strains (P < .0001), with the post hoc Dunnett multiple comparisons test compared with WT. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001; FSC-A; h, hour; IL, interleukin; LPS i.n., lipopolysaccaride intranasally; ns, nonsignificant; SSC-A, sideward scatter; TNF-α, tumor necrosis factor α.