Validation of a new mouse model of Csf3r and Asxl1 comutation. (A) Schematic of the recombination of the Csf3r-mutated allele. (B) Breeding scheme for the establishment of the Csf3r/Asxl1-mutated cohort with appropriate controls producing all following 4 groups: control, Asxl1^Y588X, Csf3r^T621I, and double mutant. (C) Polymerase chain reaction (PCR) validation of recombination of the Csf3r^T621I allele. The recombined allele is 1024 bp long; the wild type one is 947 bp. (D) Primer design for the PCR validation of Csf3r^T621I allele recombination in panel C. (E) PCR validation of the Asxl1^Y588X allele insertion. The primers used are P1 and P2 from Yang et al¹⁸ giving a 350-bp amplicon for the wild-type allele and a 570-bp amplicon for the mutated allele. (F) Western blot revealing enhanced STAT3 and STAT5 phosphorylation in the bone marrow of mice with the Csf3r^T621I allele.