HO-1 inhibition alleviates the resistance of the HbSS mice to heme. Inflammatory genes expression measured by QuanatiGene in the liver (A), heart (B), kidney (C), and lung (D) of HbAA mice or HbSS mice, pretreated or not with SnMP and injected or not by heme. Heat maps representing the average log2 FC of the different groups of mice (n = 8 mice per group for SS PBS/heme and SS SnMP/heme; n = 6 for AA PBS/PBS and SS PBS/PBS; and n = 3 for AA PBS/heme and AA SnMP/Heme). CXCL1 and CXCR2 are chemokine ligand/receptor couple that plays a crucial role in recruiting neutrophils in response to tissue injury. S100a8/S100a9 form the calprotectin, which is released by neutrophils to stimulate proinflammatory responses in hemolytic conditions. Ccl2 is a chemokine for monocytes and immune cells. Itgam is the gene coding for CD11b, marker of myeloid cells. Mrgpra2b is an MCP-counter signature gene for granulocytes. Fcgr1 is Fc gamma receptor 1, an MCP-counter signature gene for monocytes/macrophages. (E) Examples of the data used for the generation of the heat map; showing the increased expression of inflammatory genes and granulocytes infiltrate in the HbSS mice injected with SnMP and heme, compared with the HbSS mice injected with heme alone. The dotted line indicates the average level of the HbAA and HbSS mice. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; Mann-Whitney test.