Unexpected resistance of organs of HbSS mice to acute heme toxicity. Volcano plots of the signature in the liver (A), heart (B), kidney (C), lung (D), and BM (E) of HbAA mice injected with heme, compared with HbAA mice injected with PBS. (F) Common DEGs between the different organs of heme-injected HbAA mice. Volcano plots of the signature in the liver (G), heart (H), kidney (I), lung (J), and BM (K) of HbSS mice injected with heme compared with HbSS mice injected with PBS. The red dots represent the upregulated and downregulated genes in HbSS mice, compared with HbAA (adjusted P < .5; log2 FC less than or equal to −1 or ≥ 1). NS, not significant.