Figure 3.
Immune infiltrate in the HbSS organs. Evaluation of the proportion of the myeloid cells in the liver (A), heart (B), kidney (C), and lung (D), based on the expression of genes extracted from the MCP-counter signatures, measured by QuantiGene. Gating strategy (E) for evaluation by flow cytometry of the immune infiltrate on fresh whole livers (F) and kidneys (G) from HbAA mice (AA; blue) and HbSS mice (SS; red). The gating strategy was as follows: inflammatory monocytes (LD/CD45+/CD11b+CD11c–/SSC low/LY6G–/Ly6C high) (left), macrophages (LD/CD45+/CD11b+/CD11c–/LY6G–/Ly6C low) (middle), neutrophiles (LD–/CD45+/CD11b+/CD11c–/SSC high/LY6G+) (right). ∗P < .05; ∗∗P < .01. Mann-Whitney test. LD, LIVE/DEAD viability marker; mono/macro, monocytes/macrophages; ns, not significant.

Immune infiltrate in the HbSS organs. Evaluation of the proportion of the myeloid cells in the liver (A), heart (B), kidney (C), and lung (D), based on the expression of genes extracted from the MCP-counter signatures, measured by QuantiGene. Gating strategy (E) for evaluation by flow cytometry of the immune infiltrate on fresh whole livers (F) and kidneys (G) from HbAA mice (AA; blue) and HbSS mice (SS; red). The gating strategy was as follows: inflammatory monocytes (LD/CD45+/CD11b+CD11c–/SSC low/LY6G–/Ly6C high) (left), macrophages (LD/CD45+/CD11b+/CD11c–/LY6G–/Ly6C low) (middle), neutrophiles (LD–/CD45+/CD11b+/CD11c–/SSC high/LY6G+) (right). ∗P < .05; ∗∗P < .01. Mann-Whitney test. LD, LIVE/DEAD viability marker; mono/macro, monocytes/macrophages; ns, not significant.

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