Overall significant gene markers from ADE CRISPR screens. (A) CRISPR/Cas9 loss-of-function screening overall schema. (B) Significant marker genes were identified by CRISPR screens in response to AraC, Dauno, or Etop exposures, with sensitive marker genes for each drug highlighted in blue and resistant genes highlighted in red (SD cutoff, 2), whereas nonsignificant marker genes or inert marker genes highlighted in gray. (C) Top 25 sensitive and bottom 25 resistant markers for each drug screen CRISPR results. Dauno, daunorubicin; Etop, etoposide; I, inert markers for each drug; R represents resistant markers for each drug; S, sensitive markers for each drug. Diagram was created with BioRender.com.