Calcium influx and thrombin generation in PLTs from PwHAs and HCs. (A -B) Calcium influx after thrombin stimulation is significantly elevated in PwHAs when compared with HCs. (C) Thrombin generation is higher in PwHAs with increased procoagulant activity upon TRAP-6/CVX stimulation. (D) Enhanced thrombin generation (endogenous thrombin potential [ETP]) is observed in both HC and PwHA upon  thrombin receptor activating peptide-6 (TRAP-6)/CVX stimulation when compared with buffer stimulation. (E) PCP levels (CD62p+/PS+ %) positively correlate with ETP in PwHAs and HCs. (F) PCP level is significantly correlated with reduced thrombin generation lag time. Each dot represents an individual patient categorized by hemophilia severity, categorized as severe (red), moderate (yellow), mild (green), carrier (white), and HC (blue).