Megakaryopoiesis is normal in Cul5^fl/flPF4Cre mice. (A) Western blot analysis showing effective depletion of Cul5 in platelets from Cul5^fl/flPF4Cre mice. Each sample is from an independent mouse of the genotypes shown. Numbers of (B) platelets (n = 5-6), (C) megakaryocytes per field from histological sections of sternal BM (n = 6), and (D) HSCs (Lin⁻Sca1⁺Kit⁺CD150⁺CD48⁻, n = 6) in Cul5^fl/flPF4Cre and Cul5^fl/fl control mice. Each point is data from an individual mouse, bars represent mean ± SD. No statistically significant differences (P < .05) were observed for comparison of data from Cul5^fl/flPF4Cre mice to that of Cul5^fl/fl mice (unpaired Welch t test).