Mutant NPM1 MRD detection by NGS/DNA. (A) LOD of mutant NPM1 by NGS/DNA. (B) Correlation of mutant NPM1 detection by RT-qPCR/cDNA and NGS/DNA, with cases of >2% mutant NPM1/ABL1 indicated in red. (C) CIR of mutant NPM1 MRD as detected by RT-qPCR/cDNA (MRD with >2% mutant NPM1/ABL1) and/or NGS/DNA (MRD at >0.01%) in a cohort of 119 patients with mutant NPM1 AML. (D) CIR of mutant NPM1 MRD as detected by NGS/DNA in a cohort of 310 patients with mutant NPM1 AML (MRD of >0.01% VAF). Patients with detectable mutant NPM1 MRD indicated in yellow, patients without detectable mutant NPM1 MRD indicated in purple.