Analysis of liver histology. (A-B) Mice were treated with control siRNA, AT siRNA, AT siRNA + AT-WT, AT siRNA+AT-4Mut, or AT siRNA+AT-R425del as described in “Methods.” After 72 hours of siRNA treatment, mice were euthanized followed by perfusion with phosphate-buffered saline (PBS). Different organs were freshly collected and fixed with 10% neutral buffered formalin. The right lobe of the liver was collected and processed for histological analysis. Paraffin-embedded sections of the liver tissue were stained with hematoxylin and eosin and histopathological analysis was performed. Changes in the liver cytoarchitecture in animals treated with AT siRNA, with or without supplementation with AT-WT, AT-4Mut, or AT-R425del, were analyzed. Control siRNA–treated mice were used as controls. AT gene silencing (AT siRNA) leads to inflammation, characterized by leukocytic infiltration (Li) in areas surrounding the central veins (CV) or portal veins (PV). Inset boxes from each group are magnified. (C) Quantitation of leukocytes infiltration in areas surrounding the CV and PV. n ≥ 3. Scale bar, 50 μm.