AT-stimulated phagocytic activity in neutrophils. (A-B) The purified human neutrophils were labeled with calcein-AM (green) and Hoechst33342 (blue) as described in “Materials and methods.” pHrodo E coli (red) was added to neutrophils, and the phagocytosis proceeded for 60 minutes in the presence or absence of AT. Original magnification, ×20; scale bar, 25 μm. (C-D) Quantitative analysis of pHrodo E coli was performed by the total red fluorescent area (average area of pHrodo per cell, μm²/cell) obtained under different conditions.∗∗∗P < .001 vs HBSS, †††P < .001 vs HSA.