Bone marrow nonclassical monocytes are significantly reduced after GSI exposure. (A) Comparison of snRNA-Seq and snATAC-seq data using uniform manifold approximation and projection (UMAP) illustrating the automated classification of 26 distinct cell types. (B) Plasma cells were defined by their gene expression profile and the presence of aneuploidy distinguished tumor cells from normal cells. (C) Stacked bar plot comparing the cell type frequency for all samples. The percentage of nontumor cells is shown in color bars, whereas the tumor cell fraction is represented by black bars. (D) Volcano plot illustrating the results of a differential abundance analysis comparing the frequency of cell types before and after exposure to GSI. Labeled points are cell types with a P value < .05. (E) After adjustment for multiple comparisons, only nonclassical monocytes remained statistically significant, with an FDR of 0.05. DC, dendritic cell; HSC/MPP, hematopoietic stem cells/multipotent progenitor cells; pDC, plasmacytoid dendritic cell; NK, natural killer; Tcm, central memory T cell; Temra, terminally differentiated effector memory T cell; Trm, tissue-resident memory T cell.