Incorporation of α2AP into the fibrin clot. (A) 3D images of compiled Z stacks of plasma clots made using AlexaFluor 594–labeled fibrinogen (red) and AlexaFluor 488–labeled α2AP (green; scale bar, 20 μm). (B) Histograms of pixel intensity within fibrin fibers demonstrating α2AP incorporation into the clot in the presence of A11, scaffold-only Affimer, or buffer only. (C) α2AP crosslinked to fibrin with or without factor XIII (FXIII) in the presence of Affimer A11, scaffold-only Affimer control protein, or buffer only studied using an enzyme-linked immunosorbent assay. Data presented as the mean ± SD of 3 independent experiments. One-way ANOVA test was used for statistical analysis.