The effect of Affimer A11 in fibrin clot structure. Plasma clots in the presence of Affimer A11 at 10:1, 20:1, and 40:1 Affimer-to-α2AP molar ratio (scaffold-only Affimer and buffer with no Affimer were used as controls) were visualized using LSCM and scanning electron microscopy (SEM). (A) Z stacks (20.30 μm; 30 slices) were compiled to form 3-dimensional (3D) images that are presented here (scale bar, 20 μm). (B) SEM images (scale bar, 1 μm). (C) Fiber density was determined by counting the number of fibers using ImageJ software and presented as the average number of fibers per 100 μm. Two clots were made for each condition, and 3 images were taken in different areas of each clot. (D) Mean fiber thickness of fibers calculated in SEM images using ImageJ software. Two clots were made for each condition, and images were taken in 5 different areas of each clot; 20 fibers were measured in each image. Data presented as mean ± SD. Statistical analysis was performed using 1-way ANOVA; ∗P value < .05 represents difference from buffer control.