The effect of Affimer A11 on clot formation and lysis in whole blood from 3 healthy individuals using ROTEM assay and on platelet activation. (A-C) Representative trace (A), lysis time (calculated from the time of maximum clot firmness to the time of 50% reduction in maximum clot firmness; (B), and maximum clot firmness (C) from ROTEM experiments. To test the effect of Affimer A11 on platelet activation, blood was incubated with buffer, SQT, or Affimer A11 and then stimulated with thrombin. (D-E) CD62P (P-selectin) was used as platelet activation marker and presented as CD62P expression (percentage of positivity; D) and CD62P mean fluorescence intensity (E). Data are presented as the mean ± SD of 3 independent experiments. One-way ANOVA test was used for statistical analysis to compare A11 with buffer only control.