The effect of Affimer A11 on fibrin clot formation and lysis tested in turbidimetric assays. (A) Representative turbidity and lysis curves in a purified system. (B-D) The effect of Affimer A11 and control SQT on lag time (B) and maximum absorbance (C) and clot lysis time (D) of clots made from purified fibrinogen in the presence of α2AP and factor XIII. Numbers depict Affimer-to-α2AP molar ratios. (E) Representative turbidity and lysis curves in plasma. (F-H) The effect of Affimer A11 and SQT on lag time (F), maximum absorbance (G), and lysis time (H) of clots made from pooled human plasma. Data are presented as the mean ± standard deviation (SD) of 3 independent experiments performed in duplicate. One-way analysis of variance (ANOVA) test was used for statistical analysis to compare A11 with buffer only control. Max, maximum; OD, optical density.