The physicochemical structure of the CD3×CD3 BsAb. (A) schematic diagram of the IgG-scFv BsAb. (B) The purity of the CD3×CD3 BsAb was measured using high-performance liquid chromatography (HPLC). (C) The temperature stability of the CD3×CD3 BsAb was tested at 40°C over time using HPLC (percentage of the area under the curve for the main peak is shown in the diagram). n = 1 sample per time point.