Figure 5.
Tet2 does not seem to mediate the effects of ascorbate deficiency on the reconstituting potential of MPPs. Each panel in this figure compares cells from control mice, Slc23a2FL/FL;Mx1-Cre mice, Tet2FL/FL;Mx1-Cre mice, and Slc23a2FL/FL;Tet2FL/FL;Mx1-Cre mice. (A) Bone marrow cellularity in 1 tibia and 1 femur (a total of 10-11 mice per genotype in 6 independent experiments). (B) Incorporation of a 3-day BrdU pulse in HSCs and MPPs (a total of 7-9 mice per genotype in 6 independent experiments). (C) Frequencies of HSC-1, HSC-2, MPP-1, MPP-2, and MPP-3 cells in the bone marrow (a total of 10-11 mice per genotype in 6 independent experiments). (D) Donor cell reconstitution in the blood of irradiated mice competitively transplanted with 25 HSCs. For individual timepoints, ∗ indicates comparison with wild type controls and # indicates comparison with Tet2-deficient cells (a total of 6-9 recipient mice per genotype transplanted with cells from 2 donors per genotype in 2 independent experiments). (E) Donor cell reconstitution in the blood of irradiated mice competitively transplanted with 50 MPPs (a total of 15-18 recipient mice per genotype transplanted with cells from 5 donors per genotype in 5 independent experiments). (F) Summary of the donor cell reconstitution profiles in panel E. All data are presented as the mean ± standard deviation. Each dot represents a different mouse in panels A-C. Statistical significance was assessed using 1-way analysis of variance tests with Tukey's correction for multiple comparisons (A-B), Kruskal-Wallis tests with Dunn's correction for multiple comparisons (C), Kruskal-Wallis tests with Dunn's correction for multiple comparisons to assess differences among genotypes within each timepoint, and nparLD models with the false discovery rate method for multiple comparison corrections to test differences among genotypes in overall reconstitution (D-E). A Fisher exact test was used to test differences among genotypes in the number of long-term multilineage reconstituted mice (F). All statistical tests were 2-sided (∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001).

Tet2 does not seem to mediate the effects of ascorbate deficiency on the reconstituting potential of MPPs. Each panel in this figure compares cells from control mice, Slc23a2FL/FL;Mx1-Cre mice, Tet2FL/FL;Mx1-Cre mice, and Slc23a2FL/FL;Tet2FL/FL;Mx1-Cre mice. (A) Bone marrow cellularity in 1 tibia and 1 femur (a total of 10-11 mice per genotype in 6 independent experiments). (B) Incorporation of a 3-day BrdU pulse in HSCs and MPPs (a total of 7-9 mice per genotype in 6 independent experiments). (C) Frequencies of HSC-1, HSC-2, MPP-1, MPP-2, and MPP-3 cells in the bone marrow (a total of 10-11 mice per genotype in 6 independent experiments). (D) Donor cell reconstitution in the blood of irradiated mice competitively transplanted with 25 HSCs. For individual timepoints, ∗ indicates comparison with wild type controls and # indicates comparison with Tet2-deficient cells (a total of 6-9 recipient mice per genotype transplanted with cells from 2 donors per genotype in 2 independent experiments). (E) Donor cell reconstitution in the blood of irradiated mice competitively transplanted with 50 MPPs (a total of 15-18 recipient mice per genotype transplanted with cells from 5 donors per genotype in 5 independent experiments). (F) Summary of the donor cell reconstitution profiles in panel E. All data are presented as the mean ± standard deviation. Each dot represents a different mouse in panels A-C. Statistical significance was assessed using 1-way analysis of variance tests with Tukey's correction for multiple comparisons (A-B), Kruskal-Wallis tests with Dunn's correction for multiple comparisons (C), Kruskal-Wallis tests with Dunn's correction for multiple comparisons to assess differences among genotypes within each timepoint, and nparLD models with the false discovery rate method for multiple comparison corrections to test differences among genotypes in overall reconstitution (D-E). A Fisher exact test was used to test differences among genotypes in the number of long-term multilineage reconstituted mice (F). All statistical tests were 2-sided (∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001).

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