Ascorbate deficiency confers long-term reconstituting potential to quiescent MPPs. (A) Donor cell reconstitution in the blood of irradiated mice competitively transplanted with 20 H2B-GFP^high HSCs isolated from Slc23a2-deficient or littermate control mice (a total of 10-13 recipient mice per genotype transplanted with cells from 3 donors per genotype in 3 independent experiments). (B) Summary of the donor cell reconstitution profiles in panel A. (C) Donor cell reconstitution in mice competitively transplanted with 50 H2B-GFP^neg HSCs isolated from Slc23a2-deficient or littermate control mice (a total of 2-10 recipient mice per genotype transplanted with cells from 2 donors per genotype in 2 independent experiments). (D) Summary of the donor cell reconstitution profiles in panel C. (E) Donor cell reconstitution in mice competitively transplanted with 25 H2B-GFP^high MPPs isolated from Slc23a2-deficient or littermate control mice (a total of 10 recipient mice per genotype transplanted with cells from 2 donors per genotype in 2 independent experiments). (F) Summary of the donor cell reconstitution profiles in panel E. (G) Donor cell reconstitution in mice competitively transplanted with 100 H2B-GFP^neg MPPs isolated from Slc23a2-deficient or littermate control mice (a total of 6-8 recipient mice per genotype transplanted with cells from 2 donors per genotype in 2 independent experiments). (H) Summary of the donor cell reconstitution profiles in panel G. All data are presented as the mean ± standard deviation. Statistical significance was assessed using Mann-Whitney tests to assess differences among genotypes at each timepoint and nparLD models with Holm-Sidak’s multiple comparisons corrections to test differences among genotypes in the overall reconstitution (A,C,E,G). All statistical tests were 2-sided (∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001).