Ascorbate deficiency increases the self-renewal potential of HSCs and MPPs. (A-C) Donor cell reconstitution in the blood (A) and bone marrow (B-C) of irradiated mice competitively transplanted with 25 HSCs from Slc23a2-deficient or littermate control mice (a total of 15-17 recipient mice per genotype transplanted with cells from 4 donors per genotype in 4 independent experiments). (D) Donor cell reconstitution in the blood of secondary recipients of 5 million bone marrow cells from the primary recipients in panel A (a total of 7-8 recipient mice per genotype transplanted with cells from 2 donor mice per genotype in 2 independent experiments). (E-G) Donor cell reconstitution in the blood (E) and bone marrow (F-G) of irradiated mice competitively transplanted with 50 MPPs from Slc23a2-deficient or littermate control mice (we did not detect any donor HSCs in these recipients) (a total of 20-21 recipient mice per genotype transplanted with cells from 5 donors per genotype in 5 independent experiments). (H) Summary of donor cell reconstitution profiles from primary recipients of MPPs. (I-J) Donor cell reconstitution in the blood of secondary recipients of bone marrow cells from the primary recipients in panel E (a total of 10-16 recipient mice per genotype transplanted with cells from 5 donor mice per genotype in 5 independent experiments). All data are presented as the mean ± standard deviation. Each dot in panels B-C,F-G represents a different mouse. Statistical significance was assessed using Mann-Whitney tests with Holm-Sidak’s corrections for multiple comparisons (B-C,F-G), Mann-Whitney tests to assess differences among genotypes at each timepoint, and nparLD models with Holm-Sidak’s multiple comparisons corrections to test differences among overall reconstitution (A,D-E,I). All statistical tests were 2-sided (∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001).