HIV infection is associated with impaired in situ adaptive immunity in cHL TME. (A) Volcano plot showing differentially expressed genes between the 2 pathological subgroups. (B) Volcano plot showing global pathway analysis between EBV and HIV/EBV cHL tissues analyzed. (C) Dot plot diagram showing pathways enriched in the EBV or HIV/EBV cHL donors. The size of each circle corresponds to the number of genes analyzed in a given pathway (right). (D) Heat map showing top DEGs upregulated in the “downstream TCR signaling pathway” in the EBV compared with HIV/EBV cHL subgroup entity. Each column represents an individual analyzed ROI for a given donor and each row corresponds to an individual gene analyzed. (E) Volcano plot showing upregulation of specific TCR signaling–related genes in EBV compared with HIV/EBV cHL donors. All presented data were generated by the GeoMx platform. For GeoMx analysis, HIV/EBV (n = 4) and EBV cHL donors (n = 4) were analyzed. Simple linear regression for panel A and t test for panels D-E were used for the analysis. DEGs, Differentially Expressed Genes; GPCR, G Protein-Coupled Receptor; TCF, T cell factor; TGFB, Transforming Growth Factor Beta.