EBV and HIV/EBV infection are associated with similar innate immune cell composition of cHL TME. (A) Representative fluorescence images of CD11c (green), CD68 (yellow), and CD163 (magenta) in a HIV/EBV tissue (donor 22). Insets show CD11c^high (top), CD68^high (middle), and CD163^high (bottom) cells. Nuclei were visualized with DAPI. (B) Bar graphs showing the cell densities of CD11c^high, CD68^high, CD163^high, and CD68^highCD163^high in the 3 clinical pathological subgroups. (C) Representative fluorescence images of CD68 (yellow), CD163 (green), and CXCL13 (red) in a NI tissue (donor 4). Nuclei were visualized with DAPI. Insets show CD68^high (top), CD163^high (middle), and CXCL13^high (bottom) cells. (D) Bar graphs showing the cell densities of CXCL13^high, CXCL13^high CD68^high, and CXCL13^high CD163^high populations in the 3 clinical pathological subgroups. (E) Bar graph showing the cell densities of bulk CXCL13^high cells in viremic compared with ART HIV/EBV cHL TME. Statistical analyses were performed using Mann-Whitney test; ∗P < .05. (F) Representative fluorescence images of CD30 (cyan), heparan sulfate (green), and CXCL13 (red) in NI (donor 6), EBV (donor 16), and HIV/EBV (donor 27; of 4 HIV/EBV tissues analyzed) tissues. (G) Representative fluorescence images of CD8 (red) and GrzB (green) in an EBV tissue (donor 16). Nuclei were visualized with DAPI. Insets show CD8^negGrzB^high (top) and CD8^negGrzB^high (bottom) cells. (H) Bar graphs showing the cell densities of the CD8^negGrzB^high population in the 3 clinical pathological subgroups. Statistical analysis was performed using Mann-Whitney test for panels B,D,F, and P values were corrected using FDR correction with q of 0.05.