Contrary to EBV, HIV/EBV infection is associated with significantly reduced numbers of PD1^high CD4 T cells in cHL TME. (A) Representative fluorescence images of CD4 (magenta), PD-1 (orange), CD57 (yellow), and GATA3 (cyan) in an EBV tissue (donor 20). Nuclei were visualized with DAPI. (B) Bar graphs showing the cell densities of bulk CD4 as well as PD-1^high, PD-1^highGATA3^high, and PD-1^highCD57^high CD4^high T cells in the 3 clinical pathological subgroups. (C) Representative fluorescence images of CD4 (magenta), FoxP3 (red), and CD25 (cyan) in an HIV/EBV tissue (donor 21). Nuclei were visualized with DAPI. Inset shows FoxP3^highCD25^highCD4^high T cells. (D) Bar graphs showing the cell densities of CD4^highFoxP3^high and CD4^highFoxP3^highCD25^high T-cell populations in the 3 clinical pathological subgroups. The comparison between EBV and HIV/EBV cART groups is shown too (middle panel). Statistical analysis was performed using Mann-Whitney test for panels B,D, and P values were corrected using FDR correction with q of 0.05. cART, combination antiretroviral therapy.