Differential expression of coinhibitory network in EBV compared with HIV/EBV cHL. (A) Representative fluorescence images of CD8 (red), PD-1 (yellow), and TIGIT (cyan) expression in a NI tissue (donor 1). Nuclei were visualized with DAPI. Insets show a CD8 (top), PD-1^high CD8 (middle), and TIGIT^highCD8^high (bottom) cell. (B) Bar graphs showing the cell densities of bulk CD8^high and PD-1^high, TIGIT^high, and PD-1^highTIGIT^highCD8^high T cells in the 3 clinical pathological subgroups. (C) Representative fluorescence images of CD30 (cyan), Pax5 (yellow), PD-L1 (green), and CD155 (magenta) in a NI tissue (donor 6). Nuclei were visualized with DAPI. Insets show a CD155^high cell (top) and a PD-L1^high (bottom) cell. (D) Bar graphs showing the cell densities of CD30^highPD-L1^high, CD30^highCD155^high, and CD30^highPD-L1^highCD155^high populations in the 3 clinical pathological subgroups. (E) Representative fluorescence images of CD8 (red), PD-1 (yellow), GrzB (green), and CXCR3 (cyan) in an EBV tissue (donor 17). Nuclei were visualized with DAPI. Insets show a CXCR3^highCD8^high (top) and a PD-1^highGrzB^highCD8^high T cell (bottom). (F) Bar graphs depicting the cell densities of GrzB^high, GrzB^highPD-1^high, and CXCR3^highCD8^high T-cell populations in the 3 clinical pathological subgroups. Statistical analysis was performed using Mann-Whitney test for panels B,D,F, and P values were corrected using FDR correction with q of 0.05.