Viral infections do not change the cell densities of neoplastic CD30^high cells. (A) A representative hematoxylin and eosin (H&E) image showing a binucleated HRS (top arrow) and a mummified (bottom arrow) cell in an EBV tissue (donor 16) (left). Representative fluorescence image of CD30^high (cyan) staining in an EBV tissue (donor 17). Nuclei were visualized with DAPI. Inset shows a CD30^high multinuclear HRS cell (right). (B) Bar graphs showing the cell densities (left) and frequencies (right) of CD30^high cells in the 3 clinical pathological subgroups. (C) Representative fluorescence image of CD30^high (cyan), Pax5^high (yellow), and CXCR5^high (magenta) staining in a NI tissue (donor 6). Nuclei were visualized with DAPI. Insets show CD30^negPax5^high (top) and CD30^highPax5^dimCXCR5^high multinucleated HRS (bottom) cells. (D) Bar graphs depicting the cell densities (left) and frequencies (right) of CD30^highPax5^dim cells in the 3 clinical pathological subgroups. (E) Bar graphs depicting the cell densities (left) and frequencies (right) of CD30^highPax5^dimCXCR5^high cells in the 3 clinical pathological subgroups. Statistical analysis was performed using Mann-Whitney test for panels B,D-E, and P values were corrected using FDR correction with q of 0.05.