Figure 2.
Course of Adv infection and antiviral humoral immunity in a patient with myeloma after CAR T-cell therapy. (A) Time course of adenoviral copy numbers (red), ALT (alanine transaminase; blue), and AST (aspartate aminotransferase; green) serum levels. Black triangles indicate the administration of cidofovir. The black dotted line indicates the time point at which the diagnosis of Adv infection was made. (B) Time course of the anti-AdV (red) and anti-influenza A (green) titers. IgG antibody titers against full-length recombinant AdV hexon protein (Abcam, catalog no. ab123995) and influenza nucleoprotein (NP; Sino Biologicals, catalog no. 11675-V08B) were measured using enzyme-linked immunosorbent assay (ELISA). Recombinant glutathione S-transferase (GST) protein (Sino Biologicals, catalog no. 15237-H08H) was used as a control. Blue triangles indicate the administration of IV immunoglobulin. The black dotted line indicates the time point at which the diagnosis of Adv infection was made. (C) Time course of the different antimicrobial antibody titers. IgG antibody titers against full-length tetanus toxoid (green; Sigma-Aldrich, catalog no. 582231), recombinant rhinovirus (RV) capsid protein (VP1; orange; MyBioSource, catalog no. MBS1220686), varicella-zoster virus (VZV) envelope glycoprotein E (gE; red; ACROBiosystems, catalog no. GLE-V52H3), and respiratory syncytial virus (RSV) NP (purple; Sino Biologicals, catalog no. 40821-V08E) were measured by ELISA. GST protein was used as a control. Blue triangles indicate the administration of IV immunoglobulin. The black dotted line indicates the time point at which the diagnosis of Adv infection was made. (D) Time course of serum levels of total IgG (red; catalog no. BMS2091TEN) and IgM (green; catalog no. BMS2098TEN) were determined using commercially available ELISA kits from Thermo Fisher. Plasma was generated by centrifugation at 400g and frozen immediately at –80ºC. Peripheral blood mononuclear cells (PBMCs) were isolated using density gradient centrifugation and frozen in liquid nitrogen. Dx, diagnosis; LFT, liver function tests.

Course of Adv infection and antiviral humoral immunity in a patient with myeloma after CAR T-cell therapy. (A) Time course of adenoviral copy numbers (red), ALT (alanine transaminase; blue), and AST (aspartate aminotransferase; green) serum levels. Black triangles indicate the administration of cidofovir. The black dotted line indicates the time point at which the diagnosis of Adv infection was made. (B) Time course of the anti-AdV (red) and anti-influenza A (green) titers. IgG antibody titers against full-length recombinant AdV hexon protein (Abcam, catalog no. ab123995) and influenza nucleoprotein (NP; Sino Biologicals, catalog no. 11675-V08B) were measured using enzyme-linked immunosorbent assay (ELISA). Recombinant glutathione S-transferase (GST) protein (Sino Biologicals, catalog no. 15237-H08H) was used as a control. Blue triangles indicate the administration of IV immunoglobulin. The black dotted line indicates the time point at which the diagnosis of Adv infection was made. (C) Time course of the different antimicrobial antibody titers. IgG antibody titers against full-length tetanus toxoid (green; Sigma-Aldrich, catalog no. 582231), recombinant rhinovirus (RV) capsid protein (VP1; orange; MyBioSource, catalog no. MBS1220686), varicella-zoster virus (VZV) envelope glycoprotein E (gE; red; ACROBiosystems, catalog no. GLE-V52H3), and respiratory syncytial virus (RSV) NP (purple; Sino Biologicals, catalog no. 40821-V08E) were measured by ELISA. GST protein was used as a control. Blue triangles indicate the administration of IV immunoglobulin. The black dotted line indicates the time point at which the diagnosis of Adv infection was made. (D) Time course of serum levels of total IgG (red; catalog no. BMS2091TEN) and IgM (green; catalog no. BMS2098TEN) were determined using commercially available ELISA kits from Thermo Fisher. Plasma was generated by centrifugation at 400g and frozen immediately at –80ºC. Peripheral blood mononuclear cells (PBMCs) were isolated using density gradient centrifugation and frozen in liquid nitrogen. Dx, diagnosis; LFT, liver function tests.

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