Figure 6.
PO-mRNA-LNP transfection is compatible with platelet storage in that it does not adversely affect platelet quality. (A) Schematic describing platelet transfection, storage, and platelet quality assays performed. (B-C) Baseline and induced NanoLuc expression in room temperature stored platelets (RTP) (B) and cold-stored platelets (CSP) (C) transfected with PO-mRNA-LNP on day 1 (D1) and stored at 800 × 106 platelets mL−1 in PAS70:30. (D-E) Percentage of CD62P-positive RTP (D) and CSP (E). (F-G) Percentage of annexin V–positive RTP (F) and CSP (G). (H-I) Quantification of ROTEM maximum clot firmness with clotting initiated by thromboplastin in RTP (H) and CSP (I). P values were determined by the unpaired Student t-test (2-tailed). Values are reported as mean ± standard error of the mean. ∗P < .05; ∗∗∗P < .001; ∗∗∗∗P < .0001. n = 3. Ns, not significant.

PO-mRNA-LNP transfection is compatible with platelet storage in that it does not adversely affect platelet quality. (A) Schematic describing platelet transfection, storage, and platelet quality assays performed. (B-C) Baseline and induced NanoLuc expression in room temperature stored platelets (RTP) (B) and cold-stored platelets (CSP) (C) transfected with PO-mRNA-LNP on day 1 (D1) and stored at 800 × 106 platelets mL−1 in PAS70:30. (D-E) Percentage of CD62P-positive RTP (D) and CSP (E). (F-G) Percentage of annexin V–positive RTP (F) and CSP (G). (H-I) Quantification of ROTEM maximum clot firmness with clotting initiated by thromboplastin in RTP (H) and CSP (I). P values were determined by the unpaired Student t-test (2-tailed). Values are reported as mean ± standard error of the mean. ∗P < .05; ∗∗∗P < .001; ∗∗∗∗P < .0001. n = 3. Ns, not significant.

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