Structure of the D’D3/FVIII complex. (A) Three-dimensional representation of the VWF D’D3/FVIII complex derived from PDB-deposit 7KWO.²⁵ Figure was generated using PyMOL software. (B) Cartoon impression of the complex, highlighting VWF D’D3 subdomains. Principal interaction interfaces are indicated by arrows. The direct interaction between sulfated FVIII-residue Tyr¹⁶⁸⁰ and VWF D’-residue Arg⁸¹⁶ is shown as well. (C) Two-dimensional representation of the D’D3 subdomains. Circles indicate positions of residues where mutations have been associated with reduced FVIII binding in patients with von Willebrand disease-type 2N. Figure panel B adapted from Fuller et al.²⁵