CCL2 secretion from DE-DLBCL cells is crucial for macrophage recruitment and M2 polarization. MYC^high/BCL2^high, OCI-Ly8, and DOHH2 cells were cocultured with THP-1–derived macrophages in the absence or presence of anti-CCL2 nAbs. (A) MYC^high/BCL2^high cells were cocultured with THP-1–derived macrophages in a Transwell system. Macrophage migration was assessed using a migration assay (scale bar, 250 μm). (B-D) The mRNA and protein expression of macrophage differentiation markers were analyzed using qRT-PCR, IF staining, and flow cytometry (scale bar, 20 μm). The data are presented as mean ± SEM of 3 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. IFN-γ, interferon gamma; LPS, lipopolysaccharide; MFI, mean fluorescence intensity.