Upregulation of MYC/BCL2 in DLBCL cells promotes M2 polarization. The MYC^high/BCL2^high cells, OCI-Ly8 and DOHH2, and the MYC^low/BCL2^low cells, HT and SUDHL-10, were cocultured using 0.4 μm-pore size Transwells with THP-1–derived macrophages that had been differentiated with 200 μg/mL phorbol 12-myristate 13-acetate (PMA) for 24 hours. Comparative analysis of the mRNA and protein expression levels of macrophage differentiation markers in cocultured macrophages was performed using qRT-PCR and flow cytometry, respectively. (A-B) Basal MYC^high/BCL2^high and MYC^low/BCL2^low cells were cocultured with macrophages. (C) HT cells were transfected with MYC- or BCL2-expression vectors and cocultured with macrophages. (D) OCI-LY8 cells were transfected with siRNAs that targeted MYC or Bcl2 and were cocultured with macrophages. The data are presented as mean ± SEM of 3 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. IFN-γ, interferon gamma; LPS, lipopolysaccharide; MFI, mean fluorescence intensity; n.s., not significant.