Analysis of differential gene expression and variances in immune infiltration between DE-DLBCL and non–DE-DLBCL. (A) Gene set enrichment analysis revealed disparate expression patterns of gene sets when DE-DLBCL was compared with non–DE-DLBCL with a false discovery rate (FDR) >0.25. (B) A volcano plot depicting DEGs, highlighting those with an adjusted P (q) value <.05 and fold change (FC) >2. (C) Using publicly available data (Schmitz et al¹⁸), immune cell deconvolution was performed using CIBERSORTx. The number of B cells represents the combined abundance of naïve B cells, memory B cells, and plasma cells from the original CIBERSORTx data set. (D) Representative IHC images of immune cells. Immune cells stained for CD3, CD4, CD8, FOXP3, CD68, and CD163 (scale bar, 50 μm). (E) Comparative analysis of the immune cell composition between nodal DE-DLBCL and nodal non–DE-DLBCL using immunohistochemical staining and automated enumeration of immune cells. The Mann-Whitney U test was performed for panels C,E. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. NK, natural killer.