FA-GDLV–mediated oral delivery of DMT1 siRNA decreased intestinal DMT1 protein levels in Th3/+ mice. Expression of intestinal NHI transporters was assessed in 6-week-old male Th3/+ mice that had been treated with saline, empty NPs (FA-GDLVs), or with FA-GDLVs carrying negative-control (NC), or DMT1 siRNA daily for 16 days. Western blots (A) and quantitative data (B-C) are shown. For comparison, duodenal DMT1 mRNA levels are also shown (D). Data are mean ± SD for n = 3 to 5 mice per group and were analyzed by 1-way ANOVA followed by Tukey multiple comparisons test. Groups labeled with different letters are significantly different from one another. Treatment main effect: P < .001 for panels B,D.