βTI mice are anemic and iron loaded by 4 weeks of age. Shown are blood Hb (A) levels, serum EPO (B), reticulocyte count (C), serum NHI (D), liver NHI (E), serum ferritin (F), and spleen NHI levels (G) in 4- and 12-week-old male (blue circles) and female (red squares) WT and Th3/+ mice. Data are presented as mean ± SD for n = 5 mice per group and were analyzed by 3-way ANOVA. Genotype, age, and sex interactions and main effect P values are shown in each panel.