Lack of intestinal DMT1 causes iron depletion and exacerbates the anemia in Th3/+ mice. Tissue NHI levels, hematologic biomarkers, and serum hepcidin and serum EPO were assessed in male (triangles) and female (circles) littermates at 6 weeks of age. Shown are Hb (A), serum NHI (B), TSAT (C), retic count (D), serum EPO (E), liver NHI (F), spleen NHI (G), spleen weight (H), heart NHI (I), heart weight (J), serum hepcidin (K), and serum ferritin (L). Data are mean ± SD for n = 7 to 10 mice per group and were analyzed by 1-way ANOVA followed by Tukey multiple comparisons test. Groups labeled with different letters are significantly different from one another. Genotype main effects: P < .0001 for panels A,C,E-J; P < .001 for panels B,L; P < .01 for panels D,K.