Intestinal DMT1 and FPN expression is elevated in Th3/+ mice. Duodenal DMT1 and FPN protein expression was assessed in male (triangles) and female (circles) littermates at 6 weeks of age. A representative western blot for DMT1, FPN, and ACTB is shown (A), and quantitative data from all experiments are also shown (B-C). Data are mean ± standard deviation (SD) for n = 5 to 8 mice per group and were analyzed by 1-way analysis of variance (ANOVA) followed by Tukey multiple comparisons test. Groups labeled with different letters are significantly different from one another. Genotype main effects: P < .0001 for panel B; P < .01 for panel C. ACTB, beta actin.