Degradation of mtdsRNA in SD-1 conditioned medium by 100°C heat treatment prevents the IFN response and the MSC to CAF transition. (A) Fold regulation of CAF gene (IL-6, IL-8, and CCL2) and IFN pathway gene (MX2, OAS1, IFIH1, and DDX58) expression (Y axes) in HS27a cells exposed to SD-1 cell conditioned media heated to different temperatures (X axis). Bars show mean and standard error of the mean of 3 independent experiments. (B) Cytokine bead assays for IL-6, IL-8, and CCL2 (pg/mL, Y axis) following exposure of HS27a MSCs to SD-1 cell conditioned media heated to different temperatures (X axis). Mean and standard error of mean from 3 independent experiments are shown. (C) IFN-β (pg/mL, Y axis) secreted by HS27a MSCs exposed to SD-1 cell conditioned media heated to different temperatures (X axis). Three independent replicates are shown. RT, room temperature.