Association between peak plasmin level and maximum plasma clot absorbance and clot lysis time. After addition of calcium chloride (20 mM, final), phospholipids (4 μmol/L, final), TF (Innovin, 2 pmol/L final), and tPA (333 ng/mL final) to citrated plasma, turbidity was monitored by absorbance (OD) on a Thermo Scientific microplate reader. Clot lysis time was defined as the time from 50% of the peak OD during clot formation to 50% decrease in turbidity from peak OD.