C5aR1-induced inhibition of neovascularization by platelets depends on CXCL4 secretion and is distinct from platelet activation. (J-K) HLI was induced in C5aR1^−/− mice or C5aR1^−/−CXCL4^−/− mice and a WT control group, which were generated as described in the “Methods.” Double knockout mice showed no increased revascularization in comparison to C5aR1^−/− mice. Data are presented as the mean ± SEM (n = 5-7) and are displayed as a percentage of the perfusion in the contralateral control limb. n.s. = no significant difference could be observed compared with C5aR1^−/− animals. (K) Shows representative LDI images of mouse hind limbs after femoral artery ligation and during revascularization over 14 days.